Fig. 6: Pro-inflammatory cytokine “storm” in the serum after sham surgery and UNX. Serum from normal, sham surgery or UNX mice did not suppress autophagic flux in RCTE cells.
From: Surgical procedures suppress autophagic flux in the kidney
Mice underwent either no surgery, sham surgery, or UNX and were sacrificed after 2 h. A A panel of pro-inflammatory cytokines was measured in the serum: IL-1β, IL-2, IL-4, IL-6, CXCL1 (also known as IL-8 in humans and KC in mice), IL-10, IL-12, GM-CSF, IFN-γ, TNF-α. RCTE cells were treated with B normal medium or C medium containing 10% serum from normal mice, sham surgery or UNX mice. Immunoblot analysis of LC3-II and p62 in RCTE cells with or without chloroquine (CHLQ) with representative densitometry is demonstrated. Immunoblots were corrected for the endogenous control, GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001.
