Fig. 2: Necroptosis is not the main pathway related to the death of ARPE-19 cells induced by SI.
From: Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells
A Cell viabilities of ARPE-19 cells treated with 200 μM Nec-1, 2 μM NSA or 10 μM GSK′872 for 24 h before 20 mM NaIO3 treatment for 24 h (n = 4). B HT-29 cells with or without necrosulfonamide (NSA) pretreatment was treated with the indicated necrosis-inducing agents for 16 h. T + Z + S: TNF-α (200 ng/ml), Z-VAD (20 μM), and SM-164 (10 μM) (n = 4). C Phospho-RIPK1 (S166), total RIPK1, total RIPK3, Phospho-MLKL (S358), and total MLKL were detected in ARPE-19 or HT-29 cells by western blotting. SI: 10 mM NaIO3 treatment for 24 h. T + Z + S: TNF-α (100 ng/ml), Z-VAD (20 μM), and SM-164 (10 μM) treatment for 3 h. D Cell viabilities of SH-SY5Y cells treated with the indicated necrosis-inducer agent(s) for 3 h. T: 100 ng/ml TNF-α; S: 10 μM SM-164; Z: 20 μM Z-VAD (n = 4). E Cell viabilities of ARPE-19 cells treated with the indicated necrosis-inducer agent(s) for 48 h. T1: 50 ng/ml TNF-α; T2: 100 ng/ml TNF-α; S: 10 μM SM-164; Z: 20 μM Z-VAD (n = 4).
