Fig. 5: SI reacts directly with cysteine and GSH in ARPE-19 cells. | Cell Death & Disease

Fig. 5: SI reacts directly with cysteine and GSH in ARPE-19 cells.

From: Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells

Fig. 5: SI reacts directly with cysteine and GSH in ARPE-19 cells.The alternative text for this image may have been generated using AI.

A Upper panel: Western blotting detection of GPX4 expression in cells treated with 10 mM NaIO3 for the indicated time. Lower panel: the quantifications of three repeats from the upper panel. B–E The glutathione levels in ARPE-19 cells treated for 24 h with or without 10 mM NaIO3. B Total glutathione levels; C reductive glutathione levels; D oxidative glutathione levels; and E ratios of reductive to oxidative glutathione levels (n = 3). F The concentration of cysteine in ARPE-19 cells with or without 10 mM NaIO3 treatment for 24 h (n = 3). G, H In vitro reaction between SI and cysteine or GSH. 10 mM cysteine (G) or 10 mM GSH (H) was incubated with NaIO3 at indicated concentrations for 10 min at 37 °C. The residual sulfhydryl was assayed using a cysteine assay kit (n = 4).

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