Fig. 2: Depletion of RNF126 inhibited BCa cell proliferation, migration, and viability.
From: E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability
A The qRT-PCR verified the efficiency of knockdown RNF126 by siRNA (RNF126-si1 and RNF126-si2) in two BCa cells T24 and UMUC3. B The western blotting verified the expression of RNF126 downregulation in T24 and UMUC3 cells after transfection with the RNF126 siRNA. The internal control was GAPDH abundance. C, D The MTT assay evaluated the T24 and UMUC3 cells growth and viability from day 1 until day 5 after transfection with the RNF126-si1 and si2 (orange line and green line). E The colony formation assay showed the effect of RNF126 knockdown on the cell survival of UMUC3 and T24 cells after the transfection. Scale bar = 1 cm. F Colony numbers were counted and plotted as indicated. G The transwell assay evaluated cell migration of the RNF126-si1, si2 and NC treated BCa cells. Scale bar = 100 µm. H The number of cell migration was counted and statistically analyzed. I The wound healing assay evaluated the migration of siRNF126-treated BCa cells after scratching for 24 h. Scale bar = 100 µm. J The western blotting revealed the downregulation of protein abundance of EMT markers (E-Cad, N-Cad) in the BCa by reduction of RNF126. GAPDH severed as control. ***p < 0.001, **p < 0.01, *p < 0.05.
