Fig. 3: RNF126 deficiency affects the cell cycle and EGFR/PI3K/AKT signaling pathway in BCa cells.
From: E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability
A, B The flow cytometry analysis demonstrated the percentage (%) of cells in different phases of cell cycle. The T24 and UMUC3 cells were treated with RNF126-si1 and si2 for two days. C, D The percentage (%) of cells in each phase was statistically analyzed from three independent experiments. E The western blotting revealed downregulation of protein associated with the cell cycle (CCND1, CDK2 and CDK4) in the BCa cells after silencing RNF126. The internal control was GAPDH. F The relative mRNA level of CCND1 in different cell types, treatment of siRNA. H The western blotting showed the protein level of EGFR, PI3K, total and phosphorylated AKT, mTOR, and PTEN in BCa cells treated with RNF126-si1and si2. The internal control was GAPDH. I The relative mRNA level of EGFR, PI3K, AKT, PTEN in different cell types, and siRNA treatment. ***p < 0.001, **p < 0.01, *p < 0.05.
