Fig. 4: RNF126 interacts with PTEN.
From: E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability
A The co-immunoprecipitation of GFP-PTEN and FLAG-RNF126 in 293T cells. The 293T cells were transfected with FLAG-RNF126 and GFP-PTEN plasmids for 36 h. B The endogenous co-immunoprecipitation of PTEN and RNF126 in T24 cells. C–E The interactions between full-length RNF126 and truncated forms of GFP-PTEN were observed by co-immunoprecipitation in 293T cells. F The interactions between full-length PTEN and truncated forms of FLAG-RNF126 were observed by co-immunoprecipitation in 293T cells. G Protein domains of RNF126 and PTEN. Numbers indicate how long the proteins were cut off. RNF126 protein was truncated between the RING finger domain and the Zn finger domain. PTEN protein was truncated to reserve the C2 domain.
