Fig. 8: Effect of the LINC00518/miR-33a-3p/HIF-1α negative feedback loop on radiosensitivity in CMM cells in vivo. | Cell Death & Disease

Fig. 8: Effect of the LINC00518/miR-33a-3p/HIF-1α negative feedback loop on radiosensitivity in CMM cells in vivo.

From: Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1α negative feedback loop in melanoma

Fig. 8: Effect of the LINC00518/miR-33a-3p/HIF-1α negative feedback loop on radiosensitivity in CMM cells in vivo.

A Transplantation tumor experiments showing that repression of LINC00518 expression using the shLINC00518 plasmid or treating the cells with Santacruzamate A reduced the tumorigenic ability and tumor volume of WM451 and A375 cells, thereby increasing their radiosensitivity as observed by reduced colony formation in the subcutaneous sarcoma model. B Immunohistochemical assays showing that knockdown of LINC00518 expression or treating the cells with Santacruzamate A decreased HIF-1α, Ki67, and LDHA protein levels in tumor tissues of mice. C Western blotting showing that LINC00518 expression or treatment of the cells with Santacruzamate A decreased HIF-1α and LDHA protein expression in tumor tissues of mice. D Schematic of this search. The histogram data for each group are an average of three independent replicates; bars indicate SD; *P < 0.05, **P < 0.01, ***P < 0.001.

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