Fig. 6: iIC-stimulated neutrophils produce FcμRII-dependent external ROS. | Cell Death & Disease

Fig. 6: iIC-stimulated neutrophils produce FcμRII-dependent external ROS.

From: Immune complex-induced apoptosis and concurrent immune complex clearance are anti-inflammatory neutrophil functions

Fig. 6

A Following incubation with inhibitors, neutrophils were or were not stimulated with 10 μg/ml iICs for analysis of neutrophil apoptosis after 9 h. B–I Internal and total ROS production was analyzed in neutrophils that had been stimulated with 4 μg/ml iICs (B, D-I) or 25 beads/neutrophil (C) as detailed in ‘Materials and methods’. B, C Neutrophils were pre-treated for 10 min with inhibitor or vehicle as indicated prior to performing ROS assays. Internal (D, F, H) and total (E, G, I) ROS were analyzed with cells that had been pre-incubated for 30 min on ice with blocking antibodies (10.1, AT10, 3G8, ICRF44) or vehicle as indicated prior to stimulation with iICs. D–G Representative examples are shown; H, I Integrated total ROS production. A-C, H, I Each symbol represents the average value obtained in a separate experiment. A, H, I Raw data were analyzed by one-way ANOVA with multiple comparisons post-hoc test; *p < 0.05; **p < 0.01; ***p < 0.001; n.s. not significant.

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