Fig. 4: Nucleolar protein LAS1L is a target of β-catenin signaling.
From: Inhibiting β-catenin disables nucleolar functions in triple-negative breast cancer
A Schematics of workflow for TNBC cells treated with 5 µM iCRT14 for 48 h for nucleolar isolation and proteomic analysis. B Nucleolar proteome of TNBC cells (SUM1315 and MDA-MB-468) was compared with and without iCRT14 treatment. Volcanoplot reveals 19 proteins (listed) significantly reduced in nucleoli of TNBC cells after iCRT14 treatment. Special attention is drawn to LAS1L, the only protein found to be significantly altered in both TNBC vs. non-TNBC proteomics as well as the iCRT14 nucleolar proteomics. C Immunofluorescent staining of LAS1L and Fibrillarin verifying the presence of LAS1L is a nucleolar protein in TNBC cells. D Immunohistochemical staining of TNBC vs. non-TNBC tumor samples for LAS1L verifies a significant increase in LAS1L staining in the TNBC tumor samples compared to Non-TNBC tumor samples. Statistical significance was determined by T-test and error bars to represent SEM. E Diagrammatic representation of the predicted TCF consensus sequence in the promoter region of the LAS1L gene. Is a tandem overlap of two TCR/LEF sites. CCTTTGAAC is the most preferred TCF/LEF binding cite49. Most conserved bases are indicated with red color. Bases less conserved are indicated with blue and yellow colors. ChIP-qPCR analysis of β-catenin bound to the TCF consensus sequence in the promoter of the LAS1L gene. Statistical significance was determined by T-test and error bars to represent SEM.
