Fig. 5: Inflammatory changes in the skin and small intestine elicited by EC challenge were attenuated in eosinophil-deficient ΔdblGATA mice. | Cell Death & Disease

Fig. 5: Inflammatory changes in the skin and small intestine elicited by EC challenge were attenuated in eosinophil-deficient ΔdblGATA mice.

From: Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis

Fig. 5

A Hematoxylin and eosin staining of skin from the control ΔdblGATA (GATA-Con), EC-challenged ΔdblGATA (GATA-EC), and orally tolerized and EC-challenged ΔdblGATA (GATA-OT-EC) mice. The images are representative of two independent experiments. The scale bar represents 200 μm. B The thickness of the whole skin (left), epidermis (middle), and dermis (right) of the indicated mice. The graphs show the mean ± SD values. *P < 0.05, ****P < 0.0001 (one-way ANOVA). C mRNA expression of Il1b, Il1rl1, Il17a, Il25, Il33, Il5, Il13, Flg, Lor, Mcpt1, Ccr3, Prg2, and Ccl11 in the skin of the indicated mice. All data are representative of two independent experiments. The graphs show the mean ± SD values. *P < 0.05, **P < 0.01 (Student’s t-test for Il1rl1 and Il33; Mann–Whitney test for Mcpt1, Ccr3, and Prg2). D mRNA expression of Il1b, Il6, Tnf, Il17a, Il25, Il33, Il13, Cldn4, Mcpt1, Ccr3, Prg2, Ccl11, and Ccl5 in the small intestine of the indicated mice. All data are representative of two independent experiments. The graphs show the mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test for Il1b, Il6, Tnf, Il17a, and Cldn4; Mann–Whitney test for Ccr3, Prg2, and Ccl5).

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