Fig. 3: KO-miR-127-3p relieved autophagy and neurological impairment in vivo.
From: MiR-127-3p targeting CISD1 regulates autophagy in hypoxic–ischemic cortex
A Vector construction drawing of KO-miR-127-3p rats. B The examination of miR-127-3p expression in KO-miR-127-3p. F0 founders PCR screening: the molecular weight of wild type is 677 bp, # 25 missing 133 bp, # 34 missing 129 bp. F1 founders PCR screening: the molecular weight of wild type is 677 bp, # 24, 26 #, # 28 missing 133 bp, # 13, # 14, # 16 missing 129 bp. C, D TTC staining of rat brains. Images above the graph show representative slices from each group. Brains were sliced, stained with TTC, and fixed to delineate live (red) from dead or infarcted tissue (white). Infarcts were quantified by planimetry and expressed as a percentage of risk zones. Scale bar =1 cm. E Zea-longa score of rats. F Results of body weight. G, H Grip times and righting reflex time were assessed in 2 d, 3 d, 4 d, and 5 d after HI. I Time on rota rod was assessed in 22 d and 25 d after HI. J Number of crossing in 28 days. K Latency to plant forms. L Representative EM images of autophagic vacuoles were shown. Scale bar = 250 nm. The arrows depict autophagosomes. M, N Right cerebral blood flow of HI rats. O, P–R The levels of P62, ATG12, Beclin-1, LC3 I, and LC3II were detected by WB in Sham group, HI group and KO-miR-127-3p group. Data are shown as mean ± s.d. **P < 0.01 with one-way ANOVA or Kruskal–Wallis, n = 6. HI hypoxic ischemic, KO knock out, WB western blot, TTC 2,3,5-triphenyl-2H-tetrazolium chloride, D days, WT wild type, Mut mutant, NC negative control, H hours.
