Fig. 5: The detection of the direct targets of miR-127.
From: MiR-127-3p targeting CISD1 regulates autophagy in hypoxic–ischemic cortex
A Venn diagram of target genes of miR-127-3p which derived from conventional online programs of miRDB, TargetScan, miRwalk and miRmap. CISD1 and KIF3B are the common targets of miR-127-3p in the four conventional online programs which were selected for further investigation. B The alignment of the seed regions of miR-127-3p with CISD1 and KIF3B. C The wildtype and mutation alignment sequence of CISD1 and KIF3B. D Luciferase activity of CISD1 was detected at 48 h after transfection. Relative luciferase activity was calculated with (RlucmiRNA/hLuc miRNA)/(Rluc NS-miRNA/hLuc NS-miRNA). E qRT-PCR analysis of CISD1 expression at 12 and 48 h after HI injury. F, G The relative expression and concentration of CISD1 were detected. H Luciferase activity of KIF3B. I, J The relative expression and concentration of KIF3B were detected. *P < 0.05, **P < 0.01 with one-way ANOVA, n = 6.
