Fig. 7: CISD1 relieved autophagy in vitro.
From: MiR-127-3p targeting CISD1 regulates autophagy in hypoxic–ischemic cortex
A Autophagy of neurons was increased significantly in OGD, and reduced significantly in ORF-CISD1. B The relative expression of LC 3II was obviously increased in OGD, but reduced significantly in ORF-CISD1. OGD oxygen glucose deprivation. C–H The relative expression of ATG12, Beclin-1, P62, and LC 3II was obviously increased, and the relative expression of LC 3I was decreased significantly. However, the expression result of ATG12, Beclin-1, P62, LC 3I, and LC 3II was opposite in the miR127 KO rat group. Scale bar = 20 μm. Data are shown as mean ± s.d. **P < 0.01 with one-way ANOVA or Kruskal–Wallis, n = 6. OGD oxygen glucose deprivation, NC negative control, CISD1 CDGSH iron sulfurdomain-containing protein 1, ORF-CISD1 CISD1 overexpression, CISD1-NC negative control CISD1.
