Fig. 5: Acidosis enhances the expression of glucose-6-phosphate dehydrogenase to provide ribose-5-phosphate for nucleotide biosynthesis.
From: Rewiring of purine metabolism in response to acidosis stress in glioma stem cells
a Gene Set Enrichment Analysis reveals the affected purine metabolism in low-pH-treated GSC2. b Pathway enrichment analysis in response to acidosis (top 30). c Expression of stemness markers in GSC2 exposed to purine nucleotide metabolites for 48 h. d Scheme representing the main enzymes and metabolites in the pentose phosphate pathway (PPP) and de novo purine nucleotide synthesis. e Schematic representing the main enzymes in the PPP and purine nucleotide salvage synthesis. f–i Western blot analysis of the expression of the above main enzymes in GSC2, T2-4, T12-1, and LN229 under pH 6.8 or pH 7.4 treatment conditions. j, k The expression levels of H6PD and G6PD in low-grade glioma (LGG) and glioblastoma (GBM) samples using the Web-based tool GEPIA. (***p < 0.001). l, m Analysis of survival based on H6PD and G6PD expression in the glioblastoma patients by The Cancer Genome Atlas (TCGA) datasets. Log-rank test. (H6PDlow = 84; H6PDhigh = 84; G6PDlow = 84; G6PDhigh = 83).
