Fig. 3: p97 levels control stemness regulator expression, CSC population, cancer invasiveness, and mammosphere formation. | Cell Death & Disease

Fig. 3: p97 levels control stemness regulator expression, CSC population, cancer invasiveness, and mammosphere formation.

From: p97/VCP is highly expressed in the stem-like cells of breast cancer and controls cancer stemness partly through the unfolded protein response

Fig. 3: p97 levels control stemness regulator expression, CSC population, cancer invasiveness, and mammosphere formation.

a, b Immunoblotting analysis of OCT4, SOX2, and p97 in MDA-MB-231 cells with p97 silenced or MCF10A cells overexpressing p97. β-actin serves as a loading control. c, d Flow cytometry analysis of CD44+/CD24− population in MDA-MB-231 cells with p97 silenced or MCF10A cells overexpressing p97. Upper: the percentage of CD44+/CD24− cells. Lower: flow cytometry analyses. e, f Transwell invasion of MDA-MB-231 cells with p97 silenced or MCF10A cells overexpressing p97. Upper: percentage of the invaded cancer cells relative to the control group. Lower: representative images of crystal purple staining. Bar: 100 μm. g, h Mammosphere formation of MDA-MB-231 cells with p97 silenced or MCF10A cells overexpressing p97. Upper: the percentage of spheres formed under each condition relative to the control group. Lower: representative images of the spheres. Bar: 100 μm. Data were shown as mean + SD. *P < 0.05, **P < 0.01, and ***P < 0.001.

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