Fig. 4: PRRT2 affects α3-NKA clustering at the membrane surface.
From: An interaction between PRRT2 and Na+/K+ ATPase contributes to the control of neuronal excitability
A, B Representative 3D-SIM (A) and STED (B) images of hippocampal neurons (DIV 14-17) stained for α3-NKA. The images highlight an altered clustering of α3-NKA along the plasma membrane in PRRT2 KO neurons as compared to WT neurons that is rescued by expression of PRRT2 in PRRT2 KO neurons (KO + PRRT2). Scale bar, 5 μm; insets: 1 μm. C, D Size distribution of α3-NKA membrane clusters. Clusters were subdivided into bins on the basis of their size as indicated. The histograms (means ± SEM) show the area occupied by each bin of α3-NKA clusters expressed in percent of the total immunoreactive area. Both 3D-SIM (C) and STED (D) microscopy showed a significant increase in the area of largest clusters and a reciprocal decrease in the area of smallest clusters along the soma and the dendritic membrane in PRRT2 KO neurons (red bars) with respect to WT neurons (black bars). The change was fully rescued by re-expression of PRRT2 in PRRT2 KO neurons (KO + PRRT2, blue bars). Data refer to n = 35 (3D-SIM) and 25 (STED) neurons per genotype, from n = 3 independent preparations (3 coverslips/preparation/genotype). **p < 0.01, ***p < 0.001; one-way ANOVA/Bonferroni tests.
