Fig. 4: miR-21-5p expression is reduced while RUNX1 expression is raised in renal tissues of CLP rats, exosomes raise miR-21-5p expression in renal tissues of CLP rats and miR-21-5p targets RUNX1. | Cell Death & Disease

Fig. 4: miR-21-5p expression is reduced while RUNX1 expression is raised in renal tissues of CLP rats, exosomes raise miR-21-5p expression in renal tissues of CLP rats and miR-21-5p targets RUNX1.

From: Endothelial progenitor cells-derived exosomal microRNA-21-5p alleviates sepsis-induced acute kidney injury by inhibiting RUNX1 expression

Fig. 4

A/G The expression of miR-21-5p in renal tissues of CLP rats detected by RT-qPCR. B/H The expression of RUNX1 mRNA in renal tissues of CLP rats detected by RT-qPCR. C/I Protein band of RUNX1 in renal tissues of CLP rats. D/J The expression of RUNX1 protein in renal tissues of rats detected by western blot analysis. E The binding site of miR-21-5p and RUNX1 predicted by online software. F The target relationship between miR-21-5p and RUNX1 verified by dual-luciferase reporter gene assay. A–D, n = 10. *P < 0.05 vs. the sham group. #P < 0.05 vs. the Ago-NC group. &P < 0.05 vs. the Ago-miR-21-5p + Oe-NC group. G–J n = 10. *P < 0.05 vs. the CLP group. #P < 0.05 vs. the ExosAnta-NC group. F N = 3. Measurement data were depicted as mean ± standard deviation.

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