Fig. 2: USP39 promotes proliferation/invasion and epithelial-mesenchymal transition (EMT) of ovarian cancer cells. | Cell Death & Disease

Fig. 2: USP39 promotes proliferation/invasion and epithelial-mesenchymal transition (EMT) of ovarian cancer cells.

From: Splicing factor USP39 promotes ovarian cancer malignancy through maintaining efficient splicing of oncogenic HMGA2

Fig. 2

A–C Effect of USP39 on the proliferation of ovarian cancer cells was examined by (A) clonogenic, (B) growth curve and (C) EdU assays of ovarian cancer cells with USP39 overexpression (USP39) or knock down (sh-1 and sh-2) compared to corresponding control (n = 5 for clonogenic assay and n = 3 for growth curve and EdU assays). The results of these assays indicated that overexpression of USP39 promoted, whereas knockdown of USP39 prevented, the proliferation of ovarian cancer cells. D Matrigel invasion data showing that overexpression of USP39 significantly enhanced invasion capacity, whereas knockdown of USP39 (sh-1 and sh-2) decreased the invasion potential of ovarian cancer cells (n = 3 biologically independent samples). E Abundance of EMT-related markers in ovarian cancer cells with USP39 overexpression or knockdown was measured by western blot analysis. P value was obtained by Student’s t-test. Results represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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