Fig. 5: SOCS2-AS1 regulates AURKA protein stability via ubiquitination and proteasomal degradation. | Cell Death & Disease

Fig. 5: SOCS2-AS1 regulates AURKA protein stability via ubiquitination and proteasomal degradation.

From: The long-noncoding RNA SOCS2-AS1 suppresses endometrial cancer progression by regulating AURKA degradation

Fig. 5

A AURKA mRNA was determined by qPCR after SOCS2-AS1 overexpression in Ishikawa cells or knockdown in HEC-1A cells. B Immunoblotting for AURKA protein levels after SOCS2-AS1 overexpression or knockdown. GAPDH served as the internal control. C Western blot images of AURKA expression in Ishikawa cells expressing control or SOCS2-AS1 after treatment with DMSO or MG132. D Ishikawa cells with or without SOCS2-AS1 overexpression and HEC-1A cells with or without SOCS2-AS1 knockdown were treated with 25 μg/mL of cycloheximide (CHX) for the indicated time. The whole-cell lysate was detected by western blotting analysis. E Western blot images showing AURKA-associated ubiquitination in SOCS2-AS1-overexpressing cells and after the knockdown. F Immunoprecipitation to detect the association between AURKA and FBXW7 after SOCS2-AS1 overexpression in Ishikawa cells. G Immunoprecipitation to detect the association between AURKA and FBXW7 after SOCS2-AS1 knockdown in HEC-1A cells.

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