Fig. 2: FXR inhibits renal autophagy by feeding and GW4064. | Cell Death & Disease

Fig. 2: FXR inhibits renal autophagy by feeding and GW4064.

From: The critical role of FXR is associated with the regulation of autophagy and apoptosis in the progression of AKI to CKD

Fig. 2

A, B Wild-type mice were fasted for 12 h at which point were then fed a normal chow diet (A) and treated with vehicle or GW4064 for 6 h. The liver and kidneys were collected, and the mRNA levels of the indicated genes were measured by qRT-PCR. The values for fasted or vehicle treatment were set to 1 (n = 4). C Wild-type and FXR knockout mice were fasted and refed, and the kidneys were collected. The levels of autophagy-related proteins and FXR were detected by immunoblotting. The relative levels are shown (n = 3). The level of each protein, including actin, in the wild-type fasting sample was set to 1. D Wild-type and FXR knockout mice were fasted for 6 h, and the kidneys were collected. The mRNA levels of the indicated genes were measured by qRT-PCR. The values for wild-type mice were set to 1 (n = 4). All values are presented as the mean ± SD. Statistical significance was measured using one-way or two-way ANOVA with the Bonferroni post-test. *P < 0.05, **P < 0.005, ns statistically not significant, WT-Fs wild-type fasting, WT-Fd wild-type feeding, FXR KO-Fs FXR knockout fasting, FXR KO-Fd FXR knockout feeding.

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