Fig. 1: ADCK1 was upregulated in colon cancer.
A The Human Protein Atlas (HPA) database was used to analyze the correlation between ADCK1 expression and survival. The cohort was divided into two groups, high (n = 181) and low (n = 416), according to the expression of ADCK1. B The protein levels of ADCK1 in colon cancer tissues and paired normal tissues were determined. Tissues were homogenized, and proteins in the tissues were extracted using RIPA buffer. C, D IHC was performed to examine the protein levels of ADCK1 in the tissue array. Signals were detected using the Vectra 2.0 system, and statistical analysis was performed. E Survival analysis was performed. The survival curve was plotted with the Kaplan–Meier method, while the log-rank test was used for analysis. F HE staining was performed to examine the tumors in the intestines of APCmin/+ mice. Left, normal tissues; right, tumor tissues. G IHC staining was performed to examine the protein levels of ADCK1 in the tumors shown in F. The staining was scored and analyzed. The scale bars were indicated.
