Fig. 6: IL-6 and miR-155-3p-induced autophagy promote M2-like macrophage polarization via STAT3 activation. | Cell Death & Disease

Fig. 6: IL-6 and miR-155-3p-induced autophagy promote M2-like macrophage polarization via STAT3 activation.

From: Hypoxic glioma-derived exosomes promote M2-like macrophage polarization by enhancing autophagy induction

Fig. 6

A, B Human monocyte cell lines U937 and THP-1 were transfected with control or IL-6 and incubated with PMA (100 ng/ml) for 24 h in vitro to induce them to differentiate into macrophages. 3-MA was used to inhibit autophagy. S3I-201 was used to inhibit STAT3 activation. Flow cytometry and quantification were performed to analyze the proportion of CD11b + CD163 + macrophages. C, D Human monocyte cell lines U937 and THP-1 were transfected with miR-control or miR-155-3p and incubated with PMA (100 ng/ml) for 24 h in vitro to induce them to differentiate into macrophages. 3-MA was used to inhibit autophagy. S3I-201 was used to inhibit STAT3 activation. Flow cytometry and quantification were performed to analyze the proportion of CD11b + CD163 + macrophages. (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).

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