Fig. 2: CircPSMA1 promotes TNBC cell proliferation and migration and regulates cell cycle and apoptosis. | Cell Death & Disease

Fig. 2: CircPSMA1 promotes TNBC cell proliferation and migration and regulates cell cycle and apoptosis.

From: Tumor-derived exosomal circPSMA1 facilitates the tumorigenesis, metastasis, and migration in triple-negative breast cancer (TNBC) through miR-637/Akt1/β-catenin (cyclin D1) axis

Fig. 2

A The qRT-PCR analysis of circPSMA1 expression in TNBC cells transfected with circPSMA1 overexpression vector (circPSMA1) and negative control (circ-NC). B The qRT-PCR analysis of circPSMA1 expression in TNBC cells transfected with circPSMA1 siRNA vector (si-circ) and negative control (si-NC). C–E EdU assays were carried out in TNBC cells after transfection with circPSMA1 overexpression vector (circPSMA1) or circPSMA1 siRNA vector (si-circ) (magnification, ×100, Scale bar = 100 μm). F–H Colony formation assays were conducted to confirm the proliferative abilities of TNBC cells that transfected with these indicated vectors. I, J The wound healing assays were carried out to detect the migration abilities of TNBC cells after transfected with these indicated vectors (magnification, ×50, Scale bar, 200 μm). K–N Transwell assays were executed to detect the migration abilities of TNBC cells after transfected with these indicated vectors (magnification, ×100, Scale bar, 100 μm). O, P Cell cycle analysis showed that circPSMA1 could regulate TNBC cell cycle and apoptosis. Q, R The apoptotic rate of TNBC cells was analyzed by flow cytometry after downregulation of circPSMA1 expression. Data were shown as mean ± SD at least three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.

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