Fig. 5: MiR-637 inhibits TNBC cell proliferation, migration, and metastasis by directly targeted Akt1. | Cell Death & Disease

Fig. 5: MiR-637 inhibits TNBC cell proliferation, migration, and metastasis by directly targeted Akt1.

From: Tumor-derived exosomal circPSMA1 facilitates the tumorigenesis, metastasis, and migration in triple-negative breast cancer (TNBC) through miR-637/Akt1/β-catenin (cyclin D1) axis

Fig. 5

A Schematic illustration of Akt1-3′UTR-WT and Akt1-3′UTR-Mut luciferase reporter vectors and the miR-637 binding site on Akt1 that predicted by TargetScan (http://www.targetscan.org/vert_72/). B The dual-luciferase reporter assay was carried out to validate whether miR-637 could directly bind to the 3′-UTR of Akt1. After cotransfected Akt1-3′UTR-WT and Akt1-3′UTR-Mut luciferase reporter vectors with miR-637 mimic or NC respectively, the relative luciferase activities were detected by spectramax. C–G The relative expression of Akt1 after overexpression or knockdown of miR-637 by transfected with miR-637 mimic, miR-637 inhibitor, mimic-NC or inhibitor-NC was detected by qRT-PCR and western blot, respectively. H–I The relative expression of Akt1 in TNBC cells after overexpression or knockdown of circPSMA1 was detected by qRT-PCR. J–K The relative expression of Akt1 after cotransfecting the indicated vectors with miR-637 mimic, miR-637 inhibitor, mimic-NC or inhibitor-NC. Data were shown as mean ± SD at least three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.

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