Fig. 1: The key components in glycolysis pathway are highly S-nitrosylated in ovarian cancer cells. | Cell Death & Disease

Fig. 1: The key components in glycolysis pathway are highly S-nitrosylated in ovarian cancer cells.

From: The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells

Fig. 1: The key components in glycolysis pathway are highly S-nitrosylated in ovarian cancer cells.The alternative text for this image may have been generated using AI.

a The KEGG analysis for the significant enrichment pathways of 527 SNO proteins in SKOV3 cells (n = 4). b The S-nitrosylated sites of glycolytic enzymes in SKOV3 cells. c Glucose uptake and lactate production of SKOV3 cells treated with ascorbic acid (VC), GSNO, or PBS, respectively, at 37 °C for 30 min (n = 3). d Glucose uptake and lactate production of SKOV3-NOS1 knockout (NOS1-KO) vs. SKOV3 cells, and SKOV3-NOS1 over-expression (OE-NOS1) vs. SKOV3-control cells (n = 3). e Over-expression NOS1, control cells, and these SKOV3 cells treated with 2-DG (1 mM) for 48 h were subjected to CCK8 assay (n = 3). f Detect the total amount of glycolytic, TCA cycle intermediate synthesis, and amino acids in SKOV3-OE-NOS1 and control cells using GC-MS analysis technique. Each value represents the mean ± SD in three independent experiments. ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test.

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