Fig. 4: S-nitrosylation of PFKM at Cys351 promoted glucose metabolism in ovarian cancer cells.
From: The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells
a Glucose uptake and lactate production of the SKOV3-PFKM-KO cells and SKOV3-PFKM-KO+NOS1 cells which were respectively reconstituted with Flag-PFKM-WT or Flag-PFKM-C351S. b Detection of cellular glycolytic capacity (ECAR) using Seahorse XF technology in PFKM-WT and PFKM-C351S SKOV3 cells. c Detection of cellular mitochondrial oxygen consumption (OCR) using Seahorse XF technology in PFKM-WT and PFKM-C351S SKOV3 cells. d Detect the total amount of glycolytic products, TCA cycle intermediate synthesis and amino acids in PFKM-WT or PFKM-C351S SKOV3 cells using GC-MS analysis technique. e The U-13C6-glucose label and the carbon atom orientation. Relative level of glycolysis (pyruvate, alanine, serine, and lactate) and TCA cycle (citrate, α-ketoglutaric acid, succinate, glutamic acid, fumaric acid, malic acid, and aspartic acid), determined by m + 2 or m + 4 labeling of metabolites from 13C-U-glucose in PFKM-WT (pink) and PFKM-C351S (blue) SKOV3 cells (n = 3) (*P < 0.05, **P < 0.01, ***P < 0.001 by two-way ANOVA).
