Fig. 5: NRP1 is a direct target of HIF-1α. | Cell Death & Disease

Fig. 5: NRP1 is a direct target of HIF-1α.

From: HIF-1α promoted vasculogenic mimicry formation in lung adenocarcinoma through NRP1 upregulation in the hypoxic tumor microenvironment

Fig. 5

A Luciferase reporter assay in A549 and SPC-A1 cells after transfection with pGL3-reporter plasmids containing the NRP1 promoter or basic vector with or without pcDNA3.1-HIF-1α for 24 h. B, C ChIP assay was performed to detect whether HIF-1α binds to the NRP1 promoter in A549 and SPC-A1 cells. D Diagram of the NRP1 promoter structure with potential or mutant HIF-1α binding sites. E Luciferase reporter assay in A549 and SPC-A1 cells after transfection with a set of luciferase reporter plasmids containing potential binding sites of the NRP1 promoter or mutants at two HIF-1α binding sites for 24 h.

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