Fig. 8: THAP9-AS1 accelerates ESCC tumor growth in vivo. | Cell Death & Disease

Fig. 8: THAP9-AS1 accelerates ESCC tumor growth in vivo.

From: THAP9-AS1/miR-133b/SOX4 positive feedback loop facilitates the progression of esophageal squamous cell carcinoma

Fig. 8

Eca-109 cells infected with lentivirus vectors carrying sh-THAP9-AS1 or negative control (sh-NC) were inoculated into nude mice. A Growth curve of xenografts from mice in sh-NC and sh-THAP9-AS1 group. B Quantification of tumor weight in mice at 31 days after injection. C and D IHC staining and western blot assay were used to determine the protein expression of Ki-67 and PCNA in excised tumors. E qRT-PCR assay was utilized to evaluate the expression of THAP9-AS1, miR-133b, and SOX4 mRNA in resected tumors. F Western blot assay of SOX4 protein level in xenografts from mice. G A schematic illustration shows that SOX4-induced THAP9-AS1 promotes cell proliferation, migration, and invasion by sponging miR-133b and upregulating SOX4, depicting a THAP9-AS1/miR-133b/SOX4 positive feedback loop that contributes to ESCC progression. ***P < 0.001.

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