Fig. 3: Mint3 deficiency leads to decreased reactive oxygen species (ROS) production and increased cell death in LM-infected macrophages. | Cell Death & Disease

Fig. 3: Mint3 deficiency leads to decreased reactive oxygen species (ROS) production and increased cell death in LM-infected macrophages.

From: Mint3 depletion-mediated glycolytic and oxidative alterations promote pyroptosis and prevent the spread of Listeria monocytogenes infection in macrophages

Fig. 3

Production of A (ROS) and B nitric oxide (NO) in WT or Mint3–/– BMMFs. BMMFs from WT or Mint3–/– mice were infected in vitro with LM (MOI = 0.1, 1, or 10). The production of ROS and NO was measured by luminometry 8 h after LM infection and Griess assay 12 h after LM infection, respectively. Data are presented as the means ± SDs of triplicates and are representative of two independent experiments. *P < 0.05 by the Student’s t-test. C–E LM-infection-associated cell death of WT or Mint3–/– BMMFs. As a positive control, BMMFs were primed for 6 h with LPS (50 ng/mL), followed by stimulation for 2 h with nigericin (5 μM). C Cell damage/death was assessed by quantifying the lactate dehydrogenase (LDH) content of the supernatant 12 h after LM infection. Data are presented as the mean ± SD of triplicates, and are representative of two independent experiments. *P < 0.05, **P < 0.01 by Student’s t-test. D, E WT or Mint3–/– BMMFs were stained using a LIVE/DEAD Cell imaging kit 12 h after LM infection. Live (green) and dead (red) cells were discriminated using fluorescence microscopy. D Representative photographs. Scale bars = 100 μM. E Quantification of the ratio of dead cells in total cells. n = 3 per group. *P < 0.05, **P < 0.01 by Student’s t-test.

Back to article page