Fig. 3: RelA conducts as a transcriptional repressor of CXCR4 gene in EPCs. | Cell Death & Disease

Fig. 3: RelA conducts as a transcriptional repressor of CXCR4 gene in EPCs.

From: FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling

Fig. 3: RelA conducts as a transcriptional repressor of CXCR4 gene in EPCs.

A EPCs were pre-treated with CXCR4 inhibitor (AMD3100) for 30 min followed by SDF-1 stimulation for 10 min or 6 h. B EPCs were pre-treated with Erk inhibitor (PD98059), RSK inhibitor (FMK), or NF-κB inhibitor (Helenalin) for 30 min followed by SDF-1 stimulation for 10 min or 6 h. C EPCs harboring RelA or control siRNA were treated with SDF-1 for 10 min or 6 h. D WT, non-phosphorylatable S536A, or phospho-mimicking S536E mutant RelA were overexpressed in EPCs. E WT or non-phosphorylatable S536A RelA were overexpressed in EPCs followed by SDF-1 stimulation for 6 h. Protein lysates were analyzed by immunoblot using indicated antibodies. β-actin was used as a protein loading control. The numbers under the gel lanes represent the relative protein level.

Back to article page