Fig. 1: HIF-1 activated the Na+/Ca2+ exchanger brain Slc8a1 promoter. | Cell Death & Disease

Fig. 1: HIF-1 activated the Na+/Ca2+ exchanger brain Slc8a1 promoter.

From: The hypoxia sensitive metal transcription factor MTF-1 activates NCX1 brain promoter and participates in remote postconditioning neuroprotection in stroke

Fig. 1: HIF-1 activated the Na+/Ca2+ exchanger brain Slc8a1 promoter.

A Diagram showing the structure of the Rattus Norvegicus Na+/Ca2+ exchanger brain Slc8a1 promoter. HIF-1 and MTF-1 putative responsive elements (HRE-MRE) are indicated by open rectangles. The transcription start site is indicated as +1. The mRNA sequence is indicated by a black rectangle. B Luciferase assay, expressed CoCl2/CTL ratio, of SH-SY5Y cells transfected with short ncx1 (−340/+151) promoter or short HRE1mut or short HRE2mut or short HRE1-2mut, or ultrashort ncx1 promoter (−160/+151) incubated with 100 μM of CoCl2 for 24 h. *p < 0.05 vs. short ncx1 (−340/+151) promoter by one-way ANOVA followed by Bonferroni test. Each column represents the mean ± SEM of 3/10 independent experimental sessions, each one run in duplicate.

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