Fig. 4: CHOP deficiency ameliorated atherosclerotic plaque vulnerability at aortic root of ApoE−/− mice. | Cell Death & Disease

Fig. 4: CHOP deficiency ameliorated atherosclerotic plaque vulnerability at aortic root of ApoE−/− mice.

From: Intermedin1-53 attenuates atherosclerotic plaque vulnerability by inhibiting CHOP-mediated apoptosis and inflammasome in macrophages

Fig. 4

A Representative images and quantification data of CHOP immunohistochemical staining at the aortic root. Black arrows indicate the area stained positively for CHOP. Scale bars, 200 μm. n = 6. B–G Eight-week-old male ApoE−/−CHOP−/− mice and ApoE−/− littermates were fed a high-fat diet for 16 weeks. B Gross view of en face-prepared aortas stained with Oil Red O. The red dots are lesions stained positively for Oil Red O. Yellow arrows pointed to the Oil red O-stained lesions. C Representative image of Oil red O-stained aortic root lesion. Scale bars, 500 μm. D Quantification of Oil red O positive area. E Representative image of H&E-stained aortic root lesion. Thin black arrows indicate the atherosclerotic lesions. Thick black arrows indicate the necrotic core. Scale bars, 500 μm (top) and 200 μm (bottom). F Quantification of aortic root lesion area (top) and necrotic area (bottom). G, H Collagen area at the aortic root was measured. Representative picrosirius red-stained sections (G) from each group are displayed next to the quantification data (H). Scale bars, 200 μm. n = 5–11. Data are mean ± SD. *P < 0.05, **P < 0.01; Student’s t test.

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