Fig. 4: Hsa_circ_0006401 encoded a novel peptide.
From: Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma
A Schematic representation of plasmid construction. B Detection of GFP fluorescence. The indicated constructs were transfected into HeLa cells for 24 h. C Western blot analysis with anti-hsa_circ_0006401 antibodies to evaluate proteins from SW620 cells transfected with different constructs. NC (negative control), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG), siRNA (hsa_circ_0006401 circRNA level was silenced by siRNA). GAPDH was used as a loading control. D qRT-PCR analysis of hsa_circ_0006401 cirRNA expression level of different groups. E qRT-PCR analysis of hsa_circ_0006401 cirRNA expression level of different groups of SW480 cells. NC (negative control), col6a3 (col6a3 overexpression), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG). F Western blot analysis with anti-hsa_circ_0006401 antibodies (HAPL0559) to evaluate proteins from SW480 cells transfected with different constructs. NC (negative control), col6a3 (col6a3 overexpression), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG). G Reprehensive images of IHC analysis with an antibody (HAPM0617) to evaluate proteins from colon cancer tissues and normal colon tissues (left panel). IHC scores were calculated (right panel). Error bars indicate SD, *p < 0.05, **p < 0.01, ***p < 0.001.
