Fig. 1: YAP inhibits autophagy in human CRC cells. | Cell Death & Disease

Fig. 1: YAP inhibits autophagy in human CRC cells.

From: YAP inhibits autophagy and promotes progression of colorectal cancer via upregulating Bcl-2 expression

Fig. 1

A–D SW620 and HCT116 cells with stably overexpressing or knocking down of YAP were cultured in normal conditions for 24, 48, and 60 h. Total proteins were then extracted and subjected to western blot to detect the levels of the two forms of autophagy marker LC3B. E SW620 cells stably transfected with shRNA against YAP(shYAP) or scrambled sequence(shSCR) were transfected with mCherry-LC3. After 48 h of transfection, the fluorescence of mCherry-LC3 was determined under fluorescence microscope; GFP is an indicator for cells having shRNA expression. F, G Transmission electron microscopy was performed on SW620 cells with stably overexpressing or knocking down of YAP to observe autophagy. Arrowheads indicate autophagosomes formed in the cells. Data represent mean ± SD. *P < 0.05, **P < 0.01.

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