Fig. 6: Effects of nicotine on autophagy- and apoptosis-related proteins in primary cultured wild-type chondrocytes. | Cell Death & Disease

Fig. 6: Effects of nicotine on autophagy- and apoptosis-related proteins in primary cultured wild-type chondrocytes.

From: Stimulation of α7-nAChRs coordinates autophagy and apoptosis signaling in experimental knee osteoarthritis

Fig. 6

(A) Immunoblots of lysates obtained from cultured chondrocytes probed with antibodies to Beclin-1, p62, LC3, or β-actin as a loading control. (B) The MIA-induced reduction of Beclin-1 and LC3 and the increase of p62 levels were significantly decreased by 10 μM nicotine, which MLA could reverse. (C) Immunoblots of lysates obtained from cultured chondrocytes probed with antibodies against Bcl-2, Bax, cleaved caspase-3, and caspase-3. (D) The MIA-induced increase in Bax and the cleaved caspase-3 / caspase-3 ratio and the reduction of Bcl-2 were significantly decreased by 10 μM nicotine, which MLA could reverse. **P < 0.01, *P < 0.05 versus control group; ##P < 0.01, #P < 0.05 versus MIA group; $$P < 0.01 versus MIA + 10 μM nicotine by one-way ANOVA followed by Tukey’s test.

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