Fig. 5: Involvement of AP1(c-Fos/c-Jun) in the downregulation of MMP12 in MTA2-knockdown cervical cancer cells. | Cell Death & Disease

Fig. 5: Involvement of AP1(c-Fos/c-Jun) in the downregulation of MMP12 in MTA2-knockdown cervical cancer cells.

From: MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting AP1-mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis

Fig. 5: Involvement of AP1(c-Fos/c-Jun) in the downregulation of MMP12 in MTA2-knockdown cervical cancer cells.

Cells were infected with shRNA against MTA2 (shMTA2) and then subjected to (A) nucleus fractionation and immunodetection of nuclear MTA2 and AP1(c-Fos/c-Jun), (B) mRNA expression assessment via qRT-PCR, or (C) immunofluorescent detection of MTA2, c-Fos, and c-Jun by confocal microscopy. (D) Cells were transfected with luciferase reporter vectors containing the MMP12 promoters (−122/−155) or (−1801/−1793), transfected with shMTA2, and then subjected to report assay. (E) Predicted AP1 binding sites on the MMP12 promoter. (F) Cells were transfected with shMTA2 and then subjected to ChIP by using antibodies against c-Fos or c-Jun and PCR analysis. **P < 0.01 compared with shLuc cells.

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