Fig. 6: Involvement of YB1 phosphorylation in MMP12 downregulation and attenuation of metastatic potentials in MTA2-knockdown SiHa cells. | Cell Death & Disease

Fig. 6: Involvement of YB1 phosphorylation in MMP12 downregulation and attenuation of metastatic potentials in MTA2-knockdown SiHa cells.

From: MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting AP1-mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis

Fig. 6

SiHa cells were transfected with shRNA against MTA2 (shMTA2) and then subjected to (A) nucleus fractionation and immunodetection of nuclear MTA2, phosphylated YB1 (p-YB1), and YB1 or (B) immunofluorescent detection of MTA2, p-YB1, and MMP12 by confocal microscopy. (C–E) Cells were transfected with shMTA2 and siRNA against YB1 (si-YB1) and then subjected to (C) migration and invasion assay, (D) AP1 reporter assay, or (E) MMP12 mRNA assessment via qRT-PCR. F Cells were transfected with shMTA2 and then subjected to immunoprecipitation by using anti-p-YB1 antibody and immunodetection of the indicated poteins. (G, H) Cells were transfected with shMTA2 combined with siRNA against ASK1 (si-ASK1), MEK3 (si-MEK3), or p38 (si-p38) and then subjected to (G) immunodetection of the indicated proteins or (H) MMP12 mRNA expression assessment via qRT-PCR. ** and #, P < 0.01 and P < 0.05 compared with shLuc and shMTA2 cells alone, respectively.

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