Fig. 3: CXCR4 regulates DR5 transcription by differentially modulating the recruitment of transcription factors p53 and YY1 at the promoter site of DR5. | Cell Death & Disease

Fig. 3: CXCR4 regulates DR5 transcription by differentially modulating the recruitment of transcription factors p53 and YY1 at the promoter site of DR5.

From: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5

Fig. 3: CXCR4 regulates DR5 transcription by differentially modulating the recruitment of transcription factors p53 and YY1 at the promoter site of DR5.The alternative text for this image may have been generated using AI.

A Western blot analysis of DR5 in control and CXCR4 overexpressing MCF-7 cells after bafilomycin or MG132 treatment for 5 h; β-actin was used as the protein loading control. B, C Total RNA was isolated from CXCR4 overexpressing (MCF-7) and knockdown (HT-29) stable cells along with their respective controls and reverse transcribed. Fold change in DR5 mRNA expression was measured by RT-qPCR as described in Materials and Methods. Data are representative of three independent experiments, resulting from duplicate readings of two different samples; Columns, average value of DR5 mRNA expression; bars ± SEM. *, p < 0.05, compared with respective controls. D Western blot analysis of p53, YY1, and Sp1 in control and CXCR4 overexpressing MCF-7 cells; GAPDH or β-actin was used as the protein loading control. Western Blot densitometric quantification numbers are shown above the loading control blot of all immunoblot studies. E Fold change in mRNA expression of p53, YY1, and Sp1 in control and CXCR4 overexpressing stable MCF-7 cells was assessed by RT-qPCR; Columns, average value of p53/yy1/sp1 mRNA expression; bars ± SEM. *, p < 0.05, compared with respective control. F Diagrammatic representation for the p53 binding on activator site as well as YY1 binding on repressor site of the DR5 gene promoter region. G, H ChIP assay for the analysis of YY1 and p53 recruitment on the DR5 gene promoter in CXCR4 overexpressing and control stable MCF7 cells followed by RT-qPCR. Fold change in p53 and YY1 recruitment on the respective activator and repressor sites of the DR5 gene promoter were assessed in control and CXCR4 overexpressing MCF-7 cells. Results are representative of at least two independent experiments; Columns, an average of duplicate readings of samples; error bars ± S.D. *p < 0.05 versus control MCF7 cells.

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