Fig. 7: Expression of CXCR4 inversely correlates with DR5 expression in cancer cell lines, human breast cancer patient cohort and human breast cancer tissue samples. | Cell Death & Disease

Fig. 7: Expression of CXCR4 inversely correlates with DR5 expression in cancer cell lines, human breast cancer patient cohort and human breast cancer tissue samples.

From: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5

Fig. 7: Expression of CXCR4 inversely correlates with DR5 expression in cancer cell lines, human breast cancer patient cohort and human breast cancer tissue samples.The alternative text for this image may have been generated using AI.

Heat maps are displaying CXCR4 and DR5 expression in (A) breast cancer cell lines (n = 54) and in (B) GDC TCGA Breast Cancer (BRCA) patient cohort (n = 1217). Shades of red and green represent expression values in fold change. Total RNA was isolated from various cancer cell lines (C) and breast cancer patient tumor tissue samples (D), reverse transcribed, and RT-qPCR was performed for CXCR4 and DR5 expression analysis. 18 s is used as an internal control. Percentage delta Ct was determined for each sample from quadruplicate Ct value and represented in bar graph having the differential contribution of CXCR4 (red) and DR5 (green) expression. E Human formalin-fixed paraffin-embedded mammary tumor tissues were subjected to immunofluorescence staining of CXCR4 (red) and DR5 (green) proteins and analyzed by confocal microscopy. Scale bar, 5 μm sections were viewed at 63X magnification. Yellow boxed merged confocal photomicrograph area represents cell positive for DR5 (green) staining having minimal CXCR4 (red) staining.

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