Fig. 4: TRIM25 regulates EZH2 stability in CRC cells.
From: TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability
A Western blotting analysis of EZH2 in control and TRIM25-knockdown SW48 and SW480 cells. B RT-PCR analysis of EZH2 mRNA in control and TRIM25-knockdown SW48 and SW480 cells. C EZH2 protein levels in control and TRIM25-knockdown SW48 or SW480 cells were detected at the indicated time points after CHX (20 μg/mL) treatment. D The relative level of EZH2 at each time point was normalized by the level of GAPDH. E Western blotting showing the effect of MG132 (10 μM) on EZH2 levels in control and TRIM25-knockdown SW48 or SW480 cells. F Representative IHC images of TRIM25 and EZH2 in two cases of CRC. Scale bar, 100 μm. G Relationship between TRIM25 expression and EZH2 expression in the same cohort of CRC samples, as assessed using the χ2 test. H Comparison of disease-free survival between patients with different TRIM25 and EZH2 expression patterns, as assessed using the log-rank test.
