Fig. 4: HSPB3 depletion impairs the expression of gene pathways required for myogenesis, while HSPB3 overexpression induces transcriptional changes that promote myogenesis.
From: Small heat-shock protein HSPB3 promotes myogenesis by regulating the lamin B receptor
A Gene-set enrichment analysis: downregulated genes upon HSPB3 depletion in differentiating myoblasts. Analysis performed using Metascape Express Analysis on genes highly significant (P < 10−10).80 The top ten hits are shown. B Volcano plot highlighting that in differentiating myoblasts HSPB3 depletion downregulates the muscle-specific genes coding for myogenin (MYOG), actin alpha 1 (ACTA1), and desmin (DES), compared to control myoblasts. The horizontal dotted line represents P < 10−5, vertical dotted lines highlight log2 fold changes of −0.5 and 0.5. Highly significant genes (P < 10−10) with log2 fold change higher than 0.5 (or lower than −0.5) are marked in red; low significance genes (P > 10−5) with log2 fold change higher than 0.5 (or lower than −0.5) are marked in green; non-significant genes are marked in gray. C Validation by RT-qPCR of actin alpha 1 (ACTA1) and desmin (DES) downregulation in differentiated HSPB3-depleted myoblasts compared to control myoblasts. D Gene-set enrichment analysis: upregulated genes upon HSPB3 overexpression in cycling myoblasts. Analysis performed using Metascape Express Analysis on genes highly significant (P < 10−10). The top ten hits are shown. E Volcano plot highlighting that HSPB3 overexpression upregulates the matrisome genes LUM, CADM1, NID2, and DCN, as well as the SVIL and NOTCH3 genes, compared to GFP overexpression, used as a control. The horizontal dotted line represents P < 10−5, vertical dotted lines highlight log2 fold changes of −0.5 and 0.5. Highly significant genes (P < 10−10) with log2 fold change higher than 0.5 (or lower than −0.5) are marked in red; low significance genes (P > 10−5) with log2 fold change higher than 0.5 (or lower than −0.5) are marked in green; non-significant genes are marked in gray. F Validation by RT-qPCR of the genes highlighted in the volcano plot shown in C in cycling myoblast infected with lentiviral particles expressing myc-HSPB3. Related to Supplementary Fig. S4 and Supplementary Tables S1–3.
