Fig. 3: Treatments interfering with HAS3 and CD44 block autophagy flux in vitro and in vivo. | Cell Death & Disease

Fig. 3: Treatments interfering with HAS3 and CD44 block autophagy flux in vitro and in vivo.

From: Interfering with hyaluronic acid metabolism suppresses glioma cell proliferation by regulating autophagy

Fig. 3: Treatments interfering with HAS3 and CD44 block autophagy flux in vitro and in vivo.The alternative text for this image may have been generated using AI.

A TEM images of U251 glioma cells transfected with the HAS3 siRNA or treated with a CD44 antibody for 48 h. The scale bars represent 2 μm in the original image, and the scale bars represent 0.8 μm in the enlarged image. B Relative levels of the P62 and MAP1LC3B proteins in the orthotopic xenograft tumors from the control and lentivirus HAS3 shRNA or lentivirus CD44 shRNA stably transfected glioma cell groups. C Relative levels of the P62 and MAP1LC3B proteins in U251 and LN229 glioma cells transfected with the HAS3 siRNA or treated with a CD44 antibody for 48 h. D Results of the GFP-RFP-LC3 fluorescence assay using U251 glioma cells transfected with the HAS3 siRNA or treated with a CD44 antibody for 48 h. Scale bar: 10 μm. The data are presented as the mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001, ****P < 0.0001.

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