Fig. 6: 4-MU decreases glioma viability and blocks autophagy flux. | Cell Death & Disease

Fig. 6: 4-MU decreases glioma viability and blocks autophagy flux.

From: Interfering with hyaluronic acid metabolism suppresses glioma cell proliferation by regulating autophagy

Fig. 6: 4-MU decreases glioma viability and blocks autophagy flux.The alternative text for this image may have been generated using AI.

A Viability of U251 and LN229 glioma cells treated with 4-MU (1 mmol/L) for 0, 24, 48, and 72 h. Viability of U251 and LN229 glioma cells treated with different concentrations of 4-MU (0, 0.5, 1, or 2 mmol/L) for 48 h. B Levels of the Ki67 protein in LN229 glioma cells were detected using immunofluorescence staining after treatment with 4-MU for 48 h. Scale bar: 50 μm. C TEM images of U251 glioma cells treated with 4-MU for 48 h. The scale bars represent 2 µm in the original image and 0.8 μm in the enlarged image. D Relative levels of the P62 and MAP1LC3B proteins in the orthotopic xenograft tumors from the control and 4-MU-treatment groups. E Relative levels of the P62 and MAP1LC3B proteins in U251 and LN229 glioma cell lines treated with 4-MU (1 mmol/L) for 0, 3, 6, 9, and 12 h. Relative levels of the P62 and MAP1LC3B proteins in U251 and LN229 glioma cell lines treated with different concentrations of 4-MU (0, 0.5, 1, or 2 mmol/L) for 48 h. F GFP-RFP-LC3 fluorescence assays of U251 glioma cells treated with 4-MU for 48 h. Scale bar: 10 μm. The data are presented as the mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001, ****P < 0.0001.

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