Fig. 1: Mitophagic flux is increased following OGD/R. | Cell Death & Disease

Fig. 1: Mitophagic flux is increased following OGD/R.

From: Mitochondrial fission and mitophagy are independent mechanisms regulating ischemia/reperfusion injury in primary neurons

Fig. 1

A Schematic diagram of experimental design. B Utilizing primary cortical neurons from MitoQC reporter mice, cells were analyzed for the presence of mCherry puncta. Cells containing MitoQC were also stained with LAMP1, a lysosomal marker, to determine colocalization of mCherry puncta with lysosomes. C Quantification of mCherry puncta normalized to cell count. D Mander’s correlation coefficient was used to quantify colocalization of mCherry with LAMP1 throughout OGD/R. LAMP1 particle counts were analyzed and normalized to cell count. Differences across time were analyzed using one-way ANOVA with Tukey post-hoc analysis for multiple comparisons. R post-reoxygenation; *p < 0.05, **p < 0.01; ***p < 0.001 vs controls; n = 6 biological replicates. Scale bar = 10 µm.

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