Fig. 2: PINK1/Parkin-mediated mitophagy.

A Cells labeled with PINK1 and ATPB for colocalization of PINK1 with mitochondria, n = 4. B Cells labeled with Parkin and ATPB for colocalization of Parkin with mitochondria, n = 3. C Quantification of colocalization using Pearson’s correlation coefficient (PCC). D Western Blot of mitochondrial and cytosolic fractions for PINK1, Parkin, and ubiquitin. E Quantitation of protein levels, normalized to VDAC and GAPDH, Ubiquitin: n = 6, PINK1: n = 5, Parkin: n = 8. F Western Blot of LC3 (autophagy marker) and Rab5 (endosome marker) in mitochondrial and cytosolic fractions. G Quantitation of LC3 conversion and Rab5, normalized to VDAC and GAPDH, LC3: n = 4, Rab5: n = 4. Differences between groups were computed using one-way ANOVA with Dunnett’s post-hoc analysis for comparisons versus control. R post-reoxygenation; *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar = 10 µm.