Fig. 3: Mitochondria morphology classification of mCherry puncta.

A Mitochondrial morphology was classified using a machine learning-based classification system. Mitochondria were classified into four different morphologies consisting of: networks (green), unbranched (blue), swollen (yellow), and punctate (red). Scale bar = 5 µm. B mCherry puncta were segmented from images of MitoQC neurons and analyzed using our mitochondrial morphology classification system. C Total mCherry puncta counts throughout reoxygenation (R). D Stacked bar graphs representing percent red puncta throughout R. mCherry puncta: n = 4 biological replicates, 44,536 objects. Scale bar = 10 µm.