Fig. 5: Elevated p53 in the nuclei of β-catenin– MPCs. | Cell Death & Disease

Fig. 5: Elevated p53 in the nuclei of β-catenin– MPCs.

From: Wnt/ß-catenin-mediated p53 suppression is indispensable for osteogenesis of mesenchymal progenitor cells

Fig. 5

A Confocal images of anti-Runx2 and anti-Osx ICC of Ad-Cre-treated MPCs. Con: ROSA26R-Tomato. The images with dotted and solid orange lines at bottom left show magnified areas indicated by corresponding orange squares. Yellow arrows: Osx+ nuclei. White arrows: Osx+ granules. B MPCs were isolated from p53fl/fl mice and induced for osteoblastic differentiation for 7 days in the presence of DMSO (vehicle) or XAV939. ICC revealed evident β-catenin and Osx (yellow arrows) signal on vehicle-treated MPCs, whereas XAV939-treated MPCs showed no β-catenin, strong p53 nuclear staining (white arrows), and Osx signal outside of nuclei (red arrows). Only MPCs of the image labeled “+Cre” were infected with Ad-Cre. C Anti-β-catenin and anti-Osx staining of control and β-catenin– C-MPCs isolated from 3-week-old XTomato and X/CKOTomato mice. D Isolation of MPCs is detailed in Fig. 3A. The Ad-Cre-treated MPCs were cultured with or without adipogenic stimuli. Adipogenic differentiation was evaluated at day 4 by anti-Pparγ and anti-C/EBPα ICC and at d7 by Oil Red staining shown in the images with yellow outlines. Top two rows of images are taken using EVOS microscope from live cells.

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