Fig. 1: IL-4 inhibits Foxp3 transcription and histone acetylation of Foxp3 locus.

Naive CD4⁺ T cells from WT mice were cultured under Treg-polarizing conditions with or without IL-4. A Cells were harvested 3 days later and analyzed by FCM. The percentages of Foxp3⁺ cells are displayed in the quadrants. B On day 2, cells were harvested and analyzed by quantitative RT-PCR. The graphs display the relative mRNA expression of Foxp3, Gata3, Il9, and Il4. C On day 2, cells were prepared for ATAC-seq. Integrative Genomics Viewer (IGV) screenshots of representative ATAC-seq tracks from each group at the Foxp3 locus are shown. Red bands represent classic regulatory regions. The scales of the two tracks were adjusted to display comparable intensities, and the data are representative of two biologically independent pooled samples. D, E ChIP-qPCR analyses for H3Ac, H4Ac, H3K27Ac, H3K9Ac, H3K9Me2, H3K9Me3, H3K27Me2, and H3K27Me3 alterations at the Foxp3 locus were performed. Data are the mean ± SD and representative of three independent experiments. *p < 0.05.