Fig. 2: IL-4-induced Foxp3 repression is attenuated by NaB (pan-HDAC inhibitor).

Naive CD4⁺ T cells from WT B6 mice were cultured under Treg-polarizing conditions with or without IL-4 and inhibitors for 2 (D) or 3 (A, B, C) days. A The following inhibitors were added at various concentrations: trichostatin A (500 nM), NaB (250 µM), SAHA (500 nM), AES-135 (500 nM), MS-275 (250 nM), RGFP966 (2 µM), TMP269 (200 nM), CG347B (200 nM), HDAC8-IN-1 (200 nM), EX-527 (100 nM), 3-TYP (20 nM), SIRT-IN-3 (50 µM). The histogram displays the percentages of Foxp3⁺ T cells in different groups. B, C The color FACS plots depict Foxp3⁺ and GATA3⁺ cells. The percentages of different cells are displayed in the quadrants. D ChIP-qPCR assays for H3Ac, H4Ac, H3K27Ac and H3K9Ac modifications in the Foxp3 promoter, CNS1, and CNS2 regions were performed. Data are the mean ± SD and representative of three independent experiments. * p < 0.05.