Fig. 5: Hdac9 knockdown abrogates the Foxp3 suppression of IL-4.
From: IL-4 inhibits regulatory T cells differentiation by HDAC9-mediated epigenetic regulation
A ChIP-qPCR assays for HDAC9 enrichment in the Foxp3 promoter, CNS1 and CNS2 regions. B CD4+ T cells were transfected with a retrovirus carrying Hdac9-specific or Ctrl shRNA and cultured under iTreg-polarizing conditions with IL-4. On day 3, FCM assay was used to measure the percentages of Foxp3+ cells among retrovirus-transfected CD4+ T cells (GFP+). C ChIP-qPCR assays for H3Ac and H4Ac modifications in the Foxp3 promoter, CNS1 and CNS2 regions were performed with retrovirus-transfected CD4+ T cells on day 2. Data are the mean ± SD and representative of three independent experiments. *p < 0.05.
